Baculovirus Expression Technology
		Welcome to our Oxford Expression Technologies products & price list. You can buy all of the listed products by submitting a purchase order number. This section also contains further info about all of the listed products, including links to protocols, safety data, plasmid maps etc.
		For technical product data sheets please request info by mail to info@cellconcepts.de
		Genenerating recombinant Baculovirus Expression Vectors
	Article No.	Description	Size	L-Price / Size
		BacPAK6
		BacPAK6 linear DNA is the original convenient, highly efficient reagent for generating recombinant baculovirus expression vectors.  It comprises a modified Autographa californica nucleopolyhedrovirus AcMNPV) genome with lacZ inserted in place of the native polyhedrin gene for blue/white selection. Digestion of BacPAK6 DNA at three sites with Bsu36I removes lacZ and part of ORF1629, which is an essential AcMNPV gene.  The linear virus DNA is unable to initiate a replication cycle in insect cells. Cotransfection of insect cells with linear BacPAK6 DNA and a compatible transfer vector containing the complete ORF1629 and a foreign gene of interest results in recombination and restoration of the circular, infectious virus genome.  Over 95% of the virus derived from the cotransfection comprises recombinant virus.  Usually, a single plaque assay titration incubated with X-gal and counterstained with neutral red is sufficient to differentiate parental blue from recombinant white plaques.  These can be readily isolated and amplified to working stocks in a few days. The BacPAK6 reagent from OET Ltd comprises transfection-ready linearised (Bsu36I-digested) virus DNA.
	O-101101	BacPAK6	5 Reactions	352,10
	O-101102	BacPAK6	24 Reactions	949,50
	O-101103	BacPAK6	96 Reactions	3.276,50
	O-101104	BacPAK6 sec+ Linearised DNA	5 Reactions kit	352,10
	O-101105	BacPAK6 sec+ Linearised DNA	24 Reactions kit	949,50
	O-101106	BacPAK6 sec+ Linearised DNA	96 Reactions kit	3.276,50
		Production of recombinant Baculoviruses - Expression Vectors
	Article No.	Description	Size	L-Price / Size
		flashBAC
		For the production of recombinant baculoviruses. Specifically designed to remove the need to separate recombinant virus from parental virus by plaque-purification or any other means. The production of recombinant virus has been reduced to a one-step procedure  in insect cells and is thus fully amenable to high throughput and automated production systems. Baculovirus genomes contain several auxillary genes, which are non-essential for replication in insect cell culture. One of these is chitinase (chiA), which encodes an enzyme with exo- and endo chitinase activity. In an infected insect, chitinase (together with cathepsin) facilitates host cuticle breakdown and tissue liquefaction at the very late stages of infection, so releasing the virus to infect more hosts.Confocal and electron microscopy observations of insect cells infected with AcMNPV have shown that chitinase is targeted to the endoplasmic reticulum where it is densely packed in a paracrystalline array, severely compromising the function and efficacy of the secretory pathway. Deletion of chiA from flashBAC™ has improved the efficacy ofthe secretory pathway and resulted in a greatly enhanced (up to 60-fold in some instances) yield of recombinant proteins. This one-step procedure greatly facilitates the high throughput production of baculovirus expression vectors via automated systems. However, it is also of benefit to the small research group just requiring one or a few recombinant baculoviruses prepared in individual dishes of cells.
	O-100150	flashBAC	5 Reactions	231,30
	O-100151	flashBAC	24 Reactions	831,00
	O-100152	flashBAC	96 Reactions	3.156,80
	O-100153	flashBAC	Bulk	on quote
	Article No.	Description	Size	L-Price / Size
		flashBAC GOLD
		flashBACGOLD™ provides superior levels of expression for any protein that is destined for secretion or to be inserted in the membrane, or for any protein that might be particularly liable to degradation. flashBACGOLD™ is a baculovirus expression vector that has been designed to reduce proteolysis, maximise protein secretion and improve membrane protein targeting and is based on our patented flashBAC™ system, removing the necessity for plaque-purification. The deletion of both chiA and v-cath genes from flashBACGOLD™ has improved the efficacy of the secretory pathway and resulted in a greatly enhanced yield of recombinant proteins that are secreted or membrane targeted (in comparison to recombinant viruses that encode chiA and v-cath). v-cath encodes a cysteine protease. Therefore deletion of this gene results in the significant reduction of degradation of protease-sensitive targets. It is also back-compatible with all existing baculovirus transfer vectors based on homologous recombination in insect cells at the polyhedrin locus.
	O-100200	flashBAC GOLD	3 Reactions	317,90
	O-100201	flashBAC GOLD	5 Reactions	470,70
	O-100202	flashBAC GOLD	24 Reactions	1.189,00
	O-100203	flashBAC GOLD	96 Reactions	3.755,30
	O-100204	flashBAC GOLD	Bulk	on quote
	Article No.	Description	Size	L-Price / Size
		flashBAC ULTRA
		flashBACULTRA™ has taken to system a step further, by the removal of three more virus genes (p10, p74 and p26) from the flashBACULTRA™ genome. This has allowed further improvements in recombinant protein yield and quality to be delivered. Deletion of p10 increases polh activity providing more recombinant protein, increases nuclear and cellular stability, ensuring a longer timeframe for protein expression and removes a major competitor for limiting cellular resources. p74 is non-essential in cell culture but is essential for oral infectivity of occlusion-derived virus (ODV) in the host where it plays a role in midgut attachment and fusion. Deletion of p74 has been shown to have no effect on virus production in vitro. Deletion of p74 further increases the biosafety profile of recombinant baculoviruses in the environment, making them unable to traverse the insect gut wall. P26 is an early gene that codes for a 240-amino acid polypeptide of unknown function and has the same 5′ terminus as p10. Deletion of the 3’-end of p26 and fusion to lacZ or p10 have previously been shown to have no effect on virus replication in vitro. Deletion of p10, p74 and p26 removes an unnecessary genetic burden from the recombinant virus genome, providing a more efficient baculovirus expression vector. Taken together with the chitinase and cathepsin deletions this makes flashBAC ULTRA the best choice for the most difficult to express proteins. However flashBAC ULTRA is suitable for the expression of cytoplasmic, nucleus, secreted and membrane proteins.
	O-100304	flashBAC ULTRA	3 Reactions	352,10
	O-100300	flashBAC ULTRA	5 Reactions	528,80
	O-100301	flashBAC ULTRA	24 Reactions	1.401,00
	O-100302	flashBAC ULTRA	96 Reactions	4.473,60
	O-100303	flashBAC ULTRA	Bulk	on quote
	O-100400	flashBAC selection box 1.  (Includes flashBAC, flashBAC GOLD & flashBAC ULTRA)	3 x 3 Reactions	831,00
	O-100401	flashBAC selection box 2. (Includes flashBAC PRIME, flashBAC GOLD & flashBAC ULTRA)	4 x 3 Reactions	1.036,20
	Article No.	Description	Size	L-Price / Size
		flashBAC PRIME
		Designed with virus like particle production in mind, flashBAC PRIME combines the simplicity and easy to use flashBAC one step baculovirus expression system with increased recovery and yield of virus like particles. Although focused on virus like particle production, flashBAC PRIME is also well suited to cytoplasmic protein expression and production. For best results when expressing virus like particles we recommend using the recombinant virus in Trichoplusia ni (Tni) derived cell lines. The one-step procedure offered by all varients of flashBAC greatly facilitates the high throughput production of baculovirus expression vectors via automated systems. However, it is also of benefit to the small research group just requiring one or a few recombinant baculoviruses prepared in individual dishes of cells.
	O-100500	flashBAC PRIME	5 Reactions	470,70
	O-100501	flashBAC PRIME	24 Reactions	1.189,00
	O-100502	flashBAC PRIME	Bulk	on quote
		Baculovirus Protein Expression Kits
	Article No.	Description	Size	L-Price / Size
	O-400100	baculoCOMPLETE protein expression kit	5 Reactions	710,10
	O-400101	baculoCOMPLETE protein expression kit + baculoQUANT all-in-one	5 + 100 Reactions	1.121,70
		Virus Titration
	Article No.	Description	Size	L-Price / Size
	O-100602	baculoQUANT All-in-one virus extraction & titration kit	100 reacts	488,90
		Virus Expression & Titration
	Article No.	Description	Size	L-Price / Size
	O-100710	titrePLUS flashBAC all-in-one	5+100 Reactions	607,50
	O-100711	447,30	24+100 Reactions	1.144,50
	O-100712	titrePLUS flashBAC Gold all-in-one	5+100 Reactions	819,60
	O-100713	titrePLUS flashBAC Gold all-in-one	24+100 Reactions	1.469,40
	O-100714	titrePLUS flashBAC ULTRA all-in-one	5+100 Reactions	876,60
	O-100715	titrePLUS flashBAC ULTRA all-in-one	24+100 Reactions	1.657,50
	O-100716	titrePLUS flashBAC PRIME all-in-one	5+100 Reactions	819,60
	O-100717	titrePLUS flashBAC PRIME all-in-one	24+100 Reactions	1.469,40
		Transfer Plasmids - Sequencing Primers & Vectors
		The pOET1 and pOET2 plasmids are designed to allow simple, easy cloning into the baculovirus system. The pOET3 and pOET4 plasmids are designed for improved expression of glycosylated, secreted and membrane-targeted proteins and are an ideal companion to the flashBAC GOLD and ULTRA products.
	Article No.	Description (click = sequence)	Size	L-Price / Size
	O-200100	pOET sequencing primers	2 x 100 µl	101,30
	O-200101	pOET1 transfer plasmid	10 µg	208,50
	O-2001011	pOET1N_6xHis transfer plasmid	10 µg	208,50
	O-2001012	pOET1C_6xHis transfer plasmid	10 µg	208,50
	O-200103	pOET2 transfer plasmid	10 µg	208,50
	O-2001031	pOET2N/C_6xHis transfer plasmid	10 µg	208,50
	O-2001032	pOET2C_6xHis transfer plasmid	10 µg	208,50
	O-200104	pOET3 transfer plasmid	10 µg	208,50
	O-200105	pOET4 transfer plasmid	10 µg	208,50
	O-200106	pOET5 transfer plasmid	10 µg	208,50
	O-200107	pOET6 BacMAM transfer plasmid	10 µg	208,50
	O-200108	pOET1 Gateway™ vector	6 ug	403,40
	O-200109	pOET6 BacMAM Gateway® transfer plasmid	10 ug	403,40
	O-200121	pOET8.VE1 transfer plasmid	10 µg	357,80
	O-200122	pOET8.VE2 transfer plasmid	10 µg	357,80
	O-200123	pOET8.VE3 transfer plasmid	10 µg	357,80
		Transfection Reagent
	Article No.	Description	Size	L-Price / Size
	O-300105	baculoFECTIN II	150ul	140,10
	O-300106	baculoFECTIN II	1ml	470,70
		Insect Cell Culture Media
	Article No.	Description	Size	Price / 1000 ml
	O-500300	ESF921 protein free insect cell culture medium	1000 ml	109,30
	O-500301	ESF921 protein free insect cell culture medium	3x 1000 ml	76,00
	O-500302	ESF921 protein free insect cell culture medium	5x 1000 ml	69,30
	O-500304	ESF921 protein free insect cell culture medium	10x 1000 ml	64,30
	O-500305	ESF 921 serum free insect cell media	Bulk	on quote
	O-500306	Expression Systems custom made media	min 20 Ltr	on quote
	O-500307	Production Boost Additive	100 ml	104,70
	O-500308	Transfection Media	20 ml	95,60
	O-500309	Transfection Media	100 ml	169,70
	O-500310	ESF921 Delta Series Methionine deficient insect cell culture medium	1000 ml	112,70
	O-500310-50	ESF921 delta series methionine deficient insect cell culture medium	50x 1000 ml	87,70
		Insect Cells
	Article No.	Description	Size	L-Price / Size
	O-600100	Sf9 insect cells	1x10e7 cells	260,90
	O-600105	Sf21 (plaque assay) insect cells	1x10e7 cells	260,90
	O-600102	superSf9-1 insect cells	1x10e7 cells	710,10
	O-600103	superSf9-2 insect cells	1x10e7 cells	710,10
	O-600104	superSf9-3 insect cells	1x10e7 cells	710,10
	L-Prices (List-Prices) in EUR  net exw + VAT .
	Prices are subject to change without prior notice and not addressed to endusers in the legal sense of price regulation.
	Copyright © CELL CONCEPTS GmbH 1992 - 2019  &  OET - Oxford Expression Technologies 2018		08.01.2019	(vs01.19)