newsletter    2 / 2018
    cell concepts gmbh  
Surfactants for Integral Membrane Proteins  
mundenhofer weg 28   79224 umkirch  
    fon +49 7665 980410  
    fax +49 7665 980421  
     freiburg hrb 4366  
Under G-Biosciences label CELL CONCEPTS GmbH offers specially formulated reagents (detergents/surfactants) to aid the extraction, stabilization and crystallization of therapeutic integral membrane protein targets or antigens (while preserving their native three dimensional structure and function).
• No refolding
• No mutagenesis/truncation/fusion
• Striking stability improvement
• High purity
• Maintain functional and structural integrities
These reagents can be used to isolate/purify integral membrane proteins with the highest purity levels while keeping their structural and functional integrity (no refolding or mutagenesis). This has a significantly beneficial impact on the quality of the protein and on the success of the proteomics and drug discovery applications. 

We offer two varieties of innovative and customized surfactants that adapt to the biochemical characteristics of the target during the solubilization, purification/stabilization steps:
Calixarene surfactants
  These molecules are designed to structure the membrane domains of proteins through hydrophobic interactions and a network of salt bridges with the basic residues found at the cytosol-membrane interface of membrane proteins.    
Fluorinated poly(tris) and bis-glucose surfactants
Fluorinated surfactants are unable to extract proteins from membrane, but are useful for membrane protein stabilization in subsequent purification steps as they do not strip natural lipids and other co-factors from the proteins. In addition, the bulky fluorinated tails can not penetrate into the interior and disrupt structure. Fluorinated surfactants often decrease non-specific aggregation and are thought to result in improved distribution on cryo-EM grids and better vitrification for cryo-EM data collection. They are also reported to increase crystallizability. The bis-glucose compounds have a tetradhedral chiral carbon with two polar headgroups (the glucose moieties) and a long hydrophobic fluorinated tail. In this way they resemble the neo-pentyl glycol detergents, such as LMNG that have seen some recent success in structural biology.
  You may place your order either by e-m:  fon: +497665 980410  or  fax: +497665 980421    
  Sincerely yours    
  Copyright © CELL CONCEPTS GmbH 1992 - 2018.    
  All rights reserved. Any unauthorized use, reproduction or transfer of this message or its contents, is strictly prohibited published 15.061.2018