Protein Mass Spectrometry & Sequencing      
(click for =>) "MassSpec Sample Preparation Catalog of Items - Handbook & Selection Guide"
For technical product data sheets please request info by mail to info@cellconcepts.de
Cell Lysis & Protein Extraction
Article No. Description Size  L-Price / Size
PE LB - Protein Extraction & Lysis Buffer Systems 
Mild Extraction Buffers. Lysis systems for biologically active proteins
G-786-176 Bacterial PELB Kit with PELB Lysozyme 100 Preps 164,70
G-786-177 Bacterial PELB Buffer only 500 ml 119,20
G-786-178 Yeast-PELB Kit with LongLife Zymolyase 100 Preps 171,20
G-786-179 Yeast-PELBBuffer only 500  ml 119,20
G-786-180 Mammalian Cell-PELBBuffer only 500  ml 119,20
G-786-181 Tissue-PELB Buffer only 500  ml 119,20
G-786-181T Tissue-PELB; TRIAL SIZE 50  ml 28,20
G-786-411 Insect PELB™ 250 ml 119,20
FOCUS Extraction Buffers 
Denaturing Chaotropic Buffers. Chaotropic extraction buffers that preserve the
native charge of proteins
G-786-220 FOCUS™ Extraction Buffer I For 50ml 92,10
G-786-221 FOCUS™ Extraction Buffer II For 50ml 138,70
G-786-222 FOCUS™ Extraction Buffer III For 50ml 138,70
G-786-223 FOCUS™ Extraction Buffer IV For 50ml 138,70
G-786-219 FOCUS™ Extraction Buffer V For 50ml 138,70
G-786-233 FOCUSExtraction Buffer VI & DILUENT For 50ml 138,70
G-786-234 FOCUSExtraction Buffers (I-VI) & DILUENT, Trial Kit For 10 ml each Buffer 241,60
2D-Xtract™
A protein solubilization buffer for 2D analysis must solubilize
proteins effectively. 
without disturbing the native charge of the proteins
G-786-501 2D-Xtract 50 ml 138,70
FOCUS™ Proteome Kits
Isolate total proteomes from various species. FOCUS™ Proteome Kits are for the preparation of total protein, including soluble, insoluble, membrane, cytoplasmic, nuclear, signal, phospho- and glyco-proteins. The FOCUS™ Proteome Kits are suitable for biological samples from tissues, cells, plants, yeast, bacteria and insects.
G-786-246 FOCUS Mammalian Proteome 50 Preps 164,70
G-786-360 Focus Insect Proteome 50 Preps 244,90
G-786-257 FOCUS-Yeast PROTEOME 50 Preps 321,80
G-786-258 FOCUS-Bacterial PROTEOME 50 Preps 314,20
G-786-259 FOCUS-Plant PROTEOME 25 Preps 370,50
Total Protein Extraction (TPE™)
For the extraction of total protein from cells &
tissues for SDS-PAGE analysis
G-786-225 TPE™ (Total Protein Extraction) 50 Preps 110,50
RIPA Lysis & Extraction Buffer
A complete lysis buffer for the release of cytoplasmic, membrane
and nuclear proteins from adherent and suspension cultured
mammalian cells. 
G-786-489 RIPA Lysis and Extraction Buffer, [25mM Tris:HCl, 150mM NaCl, 1% NP40, 1% Sodium deoxycholate, 0.1% SDS, pH 7.6] 100 ml 61,80
G-786-490 RIPA Lysis and Extraction Buffer, [25mM Tris:HCl, 150mM NaCl, 1% NP40, 1% Sodium deoxycholate, 0.1% SDS, pH 7.6] 500 ml 134,30
 IBS - Inclusion Body Solubilization Buffers 
IBS™ buffer is specifically developed for solubilization of inclusion bodies and IBS-HP™ Buffer for the solubilization of inclusion bodies containing highly hydrophobic proteins. 
G-786-183 IBS  Buffer 100  ml 185,30
G-786-183HP IBS-HP Buffer Kit 100  ml 185,30
2D-Detergent™
Ultra low conductivity & low carbonyl & peroxide
contaminants
G-DG901 2D-DETERGENT Nonidet P-40 Substitute, 10% Aqueous Solution,  Purified, Sealed vials under argon gas 5 x 10 ml Vials 130,00
G-DG902 2D-DETERGENT Nonidet P-40 Substitute, 10% Aqueous Solution,  Purified, Sealed vials under argon gas 10 x 10 ml Vials 245,90
G-DG907 2D-DETERGENT TRITON X-100 10% Aqueous Solution,  Purified, Sealed vials under argon gas 5 x 10 ml Vials 130,00
G-DG908 2D-DETERGENT TRITON X-100 10% Aqueous Solution,  Purified, Sealed vials under argon gas 10 x 10 ml Vials 245,90
Phosphatase Inhibitor Cocktails
G-786-450 PhosphataseArrest-I [100X], Phosphatase Inhibitor Cocktail 1 ml 206,90
G-786-451 PhosphataseArrest- II [100X], Phosphatase Inhibitor Cocktail 1 ml 63,90
G-786-452 PhosphataseArrest- III [100X], Phosphatase Inhibitor Cocktail 1 ml 63,90
Protease Inhibitor Cocktails
G-786-108 ProteaseArrest [100X] 2  ml  113,80
G-786-437 ProteaseArrest™ [100X] 5 ml 232,90
G-786-711 ProteaseArrest™ [100X] 10ml 399,80
G-786-712 ProteaseArrest™ [100X] 5x10ml 1.701,00
G-786-329 OneQuant ProteaseArrest [100X] 24 x 100 µl Each 187,40
FOCUS ProteaseArrest
2D electrophoresis & mass spectrometry compatible protease inhibitor cocktail 
G-786-108F FOCUS ProteaseArrest [100X] 1  ml  173,40
High Abundant Protein Removal
Article No. Description Size  L-Price / Size
Albumin Removal
G-786-251 AlbuminOUT 25 Preps 267,60
G-786-251T AlbuminOUT Trial Size Kit 4 Preps 58,50
G-786-252 AlbuminOUT 50 Preps 493,00
Immunoglobulin G Removal
Immobilized Protein A
G-786-283 Immobilized Protein A Resin 5 ml Resin 292,50
G-786-824 Immobilized Protein A Resin 25 ml Resin 883,00
G-786-825 Immobilized Protein A Resin 5 x 1 ml columns 325,00
G-786-826 Immobilized Protein A Resin 5 columns 433,40
G-786-827 Immobilized Protein A Resin 10 x 0.2 ml columns 184,20
G-786-828 Immobilized Protein A Resin 10 columns 240,50
Immunoglobulin G Removal
Immobilized Protein G
G-786-829 Immobilized Protein G Resin 2ml Resin 227,50
G-786-284 Protein G Resin 5  ml Resin 497,30
G-786-830 Immobilized Protein G Resin 10ml Resin 922,00
G-786-832 Immobilized Protein G Resin 5 x 1ml columns 554,70
G-786-833 Immobilized Protein G Resin 5 column kit 738,90
G-786-834 Immobilized Protein G Resin 10 x 0.2ml columns 314,20
G-786-835 Immobilized Protein G Resin 10 column kit 406,30
Protein Fractionation & Enrichment
Specific Protein Enrichment
Article No. Description Size  L-Price / Size
Phosphoproteins
G-786-255 FOCUS-PhosphoRich 5 Preps 493,00
Glycoproteins
G-786-253 FOCUS-GlycoProtein  10 Preps 315,30
Membrane Proteins
G-786-249 FOCUS Membrane Proteins Extraction 50 Preps 381,40
Cell Surface Proteins
G-786-316 HOOK Cell Surface Protein Isolation kit  5 Experiments 266,50
Signal Proteins
Enrichment of signal proteins in lipid rafts
G-786-250 FOCUS Signal Protein Extraction 50 Preps 214,50
Soluble & Insoluble Proteins
A complete kit for the selective preparation of soluble (hydrophilic)
and insoluble (hydrophobic) proteins
G-786-247 FOCUS Soluble & Insoluble Protein Extraction 50 Preps 381,40
FOCUS™ Global Fractionation
Enrichment of cytosolic and membrane signal, peripheral or integral proteins
and insoluble (hydrophobic) proteins
G-786-018 FOCUS-Global Fraction Kit 50 Preps 247,00
Organelle Fractionation
Nuclear & Cytoplasmic Proteins
Fractionation of cytoplasmic and nuclear protein from cells and tissues. Supplied with a strong chaotropic extraction buffer to solubilize both cytoplasmic and nuclear proteins, which is fully compatible with   2D gel electrophoresis. 
G-786-248 FOCUS Cytoplasmic & Nuclear Protein Extraction 50 Preps 381,40
Mitochondrial Proteins
 This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of  intact inner and outer membranes and therefore retain their functionality.  
G-786-022 FOCUS Mitochondria Kit 50 Preps 273,00
Subcellular Fractionation
For the fractionation of mitochondrial, nuclear, and
cytosolic soluble and membrane proteins
G-786-260 FOCUS SuG-BCell Kit 50 Preps 370,50
General Fractionation
Fraction-FOCUS™
The high dynamic range of a species’ proteome means that the
more abundant proteins mask the less abundant and often more
interesting proteins.
Fractionation simplifies the protein composition and allows for
improved resolution and simplified 2D maps, which in turn allows
for improved analysis and interpretation and greatly increases the
chances of identifying novel and less abundant proteins.
Fraction-FOCUS™ utilizes proven technology to fractionate and
concentrate all proteomes into multiple fractions; simplifying 2D
maps and enhancing detection of low abundant proteins.
cytosolic soluble and membrane proteins
G-786-168 FractionFOCUS 10 Preps 481,10
Clean-up & Concentration
Detergent Removal Systems
Article No. Description Size  L-Price / Size
DetergentOUT™ GBS10
G-786-154 DetergentOUT™ GB-S10-125 10 columns 56,30
G-786-155 DetergentOUT™ GB-S10-800 10 columns 65,00
G-786-156 DetergentOUT™ GB-S10-3000 10 columns 170,10
G-786-157 DetergentOUT™ GB-S10-5000 10 columns 234,00
G-786-159 DetergentOUT™ GB-S10 10 ml Resin 264,40
OrgoSol DetergentOUT™
G-786-127 OrgoSol DetergentOUT, Micro For 10 ml Sample 167,90
G-786-128 OrgoSol DetergentOUT, Medi For 30 ml Sample 289,30
DetergentOUT™ Tween®
G-786-214 DetergentOUT™  Tween®, Micro 10 columns 137,60
G-786-215 DetergentOUT™  Tween®, Medi 10 columns 265,40
Clean-up & Concentration
Dialysis Systems
Article No. Description Size  L-Price / Size
Tube-O-DIALYZER™
Efficient dialysis with 100% sample recovery. For processing small samples.
G-786-610 Tube-O-DIALYZER, Micro, 1K MWCO 20 43,30
G-786-610T Tube-O-DIALYZER™, Micro, 1K MWCO 5 153,90
G-786-611 Tube-O-DIALYZER, Micro, 4K MWCO 20 43,30
G-786-611T Tube-O-DIALYZER™, Micro 4K MWCO 5 153,90
G-786-612 Tube-O-DIALYZER, Micro, 8K MWCO 20 43,30
G-786-612T Tube-O-DIALYZER™, Micro 8K MWCO 5 153,90
G-786-613 Tube-O-DIALYZER, Micro, 15K MWCO 20 43,30
G-786-613T Tube-O-DIALYZER™, Micro 15K MWCO 5 153,90
G-786-614 Tube-O-DIALYZER, Micro, 50K MWCO 20 43,30
G-786-614T Tube-O-DIALYZER™, Micro 50K MWCO 5 172,30
G-786-615 Tube-O-DIALYZER, Medi, 1K MWCO 20 49,80
G-786-615T Tube-O-DIALYZER™, Medi, 1K MWCO 5 172,30
G-786-616 Tube-O-DIALYZER, Medi, 4K MWCO 20 49,80
G-786-616T Tube-O-DIALYZER™, Medi, 4K MWCO 5 172,30
G-786-617 Tube-O-DIALYZER, Medi, 8K MWCO 20 49,80
G-786-617T Tube-O-DIALYZER™, Medi, 8K MWCO 5 172,30
G-786-618 Tube-O-DIALYZER, Medi, 15K MWCO 20 49,80
G-786-618T Tube-O-DIALYZER™, Medi, 15K MWCO 5 172,30
G-786-619 Tube-O-DIALYZER, Medi, 50K MWCO 20 49,80
G-786-619T Tube-O-DIALYZER™, Medi, 50K MWCO 5 166,90
G-786-620 Tube-O-DIALYZER, Mixed, 1K MWCO 20 166,90
G-786-621 Tube-O-DIALYZER, Mixed, 4K MWCO 20 166,90
G-786-622 Tube-O-DIALYZER, Mixed, 8K MWCO 20 166,90
G-786-623 Tube-O-DIALYZER, Mixed, 15K MWCO 20 166,90
G-786-624 Tube-O-DIALYZER, Mixed, 50K MWCO 20 153,90
Clean-up & Concentration
Contamination Removal
Article No. Description Size  L-Price / Size
Spin-OUT™ Desalting Columns
For desalting and buffer exchange 
G-786-170 SpinOUT GT-600, Micro 10 Columns 58,50
G-786-171 SpinOUT GT-600, Medi 10 Columns 92,10
G-786-172 SpinOUT GT-1200, Micro 10 Columns 58,50
G-786-173 SpinOUT GT-1200, Medi 10 Columns 92,10
G-786-703 Spin-OUT GT-600, 0.1ml 25 Columns 123,50
G-786-704 Spin-OUT GT-600, 5ml 5 Columns 101,90
G-786-705 Spin-OUT GT-600, 10ml 5 Columns 151,70
G-786-706 Spin-OUT GT-1200, 0.1ml 25 Columns 123,50
G-786-707 Spin-OUT GT-1200, 5ml 5 Columns 85,60
G-786-708 Spin-OUT GT-1200, 10ml 5 Columns 115,90
Clean-up & Concentration
Concentration Systems
Article No. Description Size  L-Price / Size
UPPA-PROTEIN-Concentrate™
To quantitatively concentrate dilute protein samples as
low as 1ng/ml. Concentration is not affected by the presence of
common laboratory agents, including detergents and chaotropes 
G-786-120 UPPA PROTEIN Concentrate, Micro For 10 ml Sample 308,80
G-786-121 UPPA PROTEIN Concentrate, Medi For 30 ml Sample 482,10
G-786-122 UPPA-I & UPPA-II Pack,  250ml each For >80 ml  138,70
OrgoSol-PROTEIN-Concentrate™
Preserve biological activity during concentration 
G-786-125 OrgoSol™ PROTEIN Concentrate For 5  ml Protein  136,50
Column-PROTEIN-Concentrate™
For larger volumes of dilute protein solutions
G-786-126 Column PROTEIN Concentrate For 4 mg protein 136,50
Tube-O-CONCENTRATOR™
Rapid concentration without protein precipitation, utilizes a novel, water absorbing, liquid polymer and our patented Tube-O-DIALYZER™ for the rapid concentration of dilute protein solutions with zero protein loss
G-786-625 Tube-O-CONCENTRATOR™ for 20-250μl volumes 5 153,90
G-786-626 Tube-O-CONCENTRATOR™ for 0.2-2.5ml volumes 5 180,90
Clean-up & Concentration
Electrophoresis Clean Up
Article No. Description Size  L-Price / Size
PAGE-Perfect™
PAGE-Perfect™ is a simple, two-step method for concentrating,
cleaning and preparing protein solutions for running publication
quality gels
G-786-123 PAGE-Perfect 50 Preps 256,80
G-786-123T PAGE-Perfect, Trial Size 5 Preps 45,50
Perfect-FOCUS™
Streak free 2D gels & improved spot resolution 
G-786-124 Perfect FOCUS 50 Preps 250,30
G-786-124T Perfect FOCUS Kit, Trial Size 6 Preps 41,20
Electrophoresis Separation
Protein Markers
Article No. Description Size  L-Price / Size
PAGEmark™ Protein Ladder
The unstained and prestained PAGEmark™ Markers are ~7 to
>210kDa* ladder of eight proteins. The prestained PAGEmark™
proteins are covalently coupled to a blue dye and PAGEmark™ Tricolor
proteins consist of six coupled to a blue dye, one to an orange
dye and one to a red dye for easy reference
G-786-413 PAGEmark™ Unstained Marker 100 Loads 34,70
G-786-414 OneQuant™ PAGEmark™ Unstained Marker 40 Loads 44,40
Electrophoresis Separation
Protein Reduction & Alkylation
Article No. Description Size  L-Price / Size
FOCUS™ Protein Alkylation
The kit is supplied with a proprietary buffer necessary for efficient
alkylation of thiols, while minimizing reoxidation of the competing
thiol pairs in protein samples
G-786-230 FOCUSProtein Reductant Kit 100 Preps 130,00
G-786-231 FOCUSProtein Reduction-Alkylation Kit 100 Preps 214,50
G-786-232 FOCUS Protein Alkylation Kit 100 Preps 130,00
FOCUS DTT 
Ultra pure proteomic grade DTT for protein reduction
G-786-227 FOCUS DTT (2M) 5 x 1 ml 69,30
Iodoacetamide
Streak free 2D gels & improved spot resolution 
G-786-228 Iodoacetamide 5 gr 56,10
G-RC-150 Iodoacetamide 25 gr 258,70
G-786-078 OneQuant Iodoacetamide,5 strips of 8 vials  each, 15 mg / Vial 40 Vials 126,80
Electrophoresis Separation
Mass Spectrometry Compatible Protein Gel Stains
Article No. Description Size  L-Price / Size
LabSafe GEL Blue™
Ultra-sensitive, fast, ready-to-use protein stain 
G-786-35 LabSafe GEL Blue - Ultra-sensitive, fast, ready-to-use enhanced protein stain 1 Ltr 94,30
G-786-35G LabSafe GEL Blue - Ultra-sensitive, fast, ready-to-use enhanced protein stain 1 Gal 227,50
FOCUS™ FASTsilver™
Mass spectrometry compatible silver stain for
enhanced spot visualization
G-786-240 FOCUS FASTsilver Kit - Mass Spectrometry compatible silver staining for enhanced spot visualisation  25 Mini Gels 210,20
G-786-240T FOCUS FASTsilver Kit - Mass Spectrometry compatible silver staining for enhanced spot visualisation  5 Mini Gels 59,60
Reversible Zinc Stain™
Stains hard to stain proteins, including
glycoproteins & phosphoproteins
G-786-32ZN Reversible Zinc Stain - For hard-to-stain proteins, including glycoproteins & phosphoproteins 25 Mini Gels 171,20
G-786-32DSZN Destaining Solution-Zinc (10x) 500 ml 171,20
RUBEO™ Fluorescent Protein Stain
RUBEO™ is a fluorescent protein stain that is ideal for staining
proteins in 1D and 2D electrophoresis gels. The fluorescent
stain has a red emission at 605nm. The fluorescent stain has
nanogram sensitivity and is compatible with mass spectroscopy,
microsequencing and immunostaining
G-786-644 RUBEO Fluorescent Protein Stain 200ml 54,20
G-786-645 RUBEO Fluorescent Protein Stain 1L 190,70
Peptide Generation
Trypsin
Article No. Description Size  L-Price / Size
Trypsin for Mass Spectrometry
Trypsin is a serine endopeptidase that specifically cleaves peptide
bonds on the carboxy side of s-aminoethyl cysteine, arginine and
lysine residues
G-786-245P MSG Trypsin (Porcine), MassSpec Sequencing Grade 5 vials a 20ug 57,40
G-786-245B MSG Trypsin (Bovine), MassSpec Sequencing Grade 5 vials a 20ug 57,40
G-786-687P Trypsin (Porcine), Mass Spectrometry Grade 100 µg 55,30
G-786-687B Trypsin (Bovine), Mass Spectrometry Grade 100µg 55,30
G-786-688P Trypsin (Porcine), Mass Spectrometry Grade 200 µg 104,00
G-786-690 Trypsin (Porcine), Mass Spectrometry Grade 5 x 100µg 237,30
G-786-693 Trypsin (Porcine), Mass Spectrometry Grade 5 x 200µg 459,40
Peptide Generation
Sequencing Grade Proteases
Article No. Description Size  L-Price / Size
SG-Chymotrypsin
A serine endopeptidase, which predominantly cleaves peptide bonds on the carboxy side of tyrosine, phenylalanine and tryptophan
G-786-13 SG-Chymotrypsin 2 x 25 µg 162,50
SG-Lysine-C™
An endopeptidase, from Lysobacter enzymogenes, is a serine protease highly specific in cleaving peptide bonds at the carboxy side of lysine
G-786-14 SG-Lysine-C 2 x 5 µg 417,10
SG-Glutamic-C™
A serine endopeptidase, from Staphylococcus aureus V8, that
is highly specific for the cleavage of peptide bonds at the carboxy
side of either aspartic or glutamic acid, depending on the buffer
used
G-786-15 Protease SG™-Glutamic-C 2 x 10 µg 153,90
SG-Arginine-C™
An endopeptidase (Clostripain, from Clostridium histolyticum)
specifically hydrolyzes the carboxy peptide bond of Arginine
G-786-11 SG Protease Arginine-C 2 x 5 µg 167,90
Immobilized Trypsin
Immobilized Trypsin is TPCK treated trypsin immobilized on 4%
agarose that eliminates the contamination of protein digests by the
trypsin
G-786-792 Immobilized Trypsin 2 ml Resin 92,10
Peptide Generation - InGel Digestion
Complete sets of reagents for the in gel tryptic digestion and extraction of peptides for Mass Spectrometry  (MALDI & LC MS/MS).
Article No. Description Size  L-Price / Size
G-786-241 InGel Silver - In gel digestion of proteins in silver stained gels 100 Preps 210,20
G-786-681 InGel Blue - In gel digestion of proteins in Coomassie and fluorescent stained gels 100 Preps 196,10
G-786-241A InGel Array – 96 Well format for high throughput in gel digestion of protein spots 500 Preps 493,00
Accessories
Article No. Description Size  L-Price / Size
G-786-244 SilverOUT - for removal of silver stain 100 Preps 136,50
G-786-683 Blue-OUT - for removal of coomassie stain 10 ml 92,10
G-786-243 Pep-Extract - Peptide extraction Buffer (MassSpec Grade).  100 Preps 136,50
Following in gel digestion of proteins with proteases, the addition of Pep-Extract™ rapidly elutes digested peptides in under 20 minutes.  The eluted peptides can be used directly in mass spectrometry analysis, without any additional clean up
G-786-242 Trypsin-Digestion Buffer (MassSpec Grade) 100 Preps 136,50
G-786-229 Proteomic Grade Water, ultra pure 1L 30,30
G-786-680 Protein-OUT™ - Mass spectrometry compatible washing solution 50 ml 89,90
G-786-300 Proteomic Grade Microfuge Tubes 100 Tubes 57,40
  L-Prices (List-Prices) in EUR  net exw + VAT .      
  Prices are subject to change without prior notice and not addressed to endusers in the legal sense of price regulation.  
           
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