|
|
|
|
|
|
|
|
Baculovirus Expression Technology |
|
|
|
|
|
|
|
|
| |
2022
PRICE LIST - CELL CONCEPTS /
OXFORD EXPRESSION TECHNOLOGIES |
|
|
|
|
|
|
|
|
|
For
technical product data sheets please request info by mail to info@cellconcepts.de |
|
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
Genenerating recombinant Baculovirus
Expression Vectors |
|
|
|
|
|
|
|
|
Article No. |
Description |
Size |
Price |
|
|
|
|
|
|
|
|
|
BacPAK6 |
|
|
|
|
|
BacPAK6 linear DNA is the
original convenient, highly efficient reagent for generating recombinant
baculovirus expression vectors. It
comprises a modified Autographa californica nucleopolyhedrovirus AcMNPV)
genome with lacZ inserted in place of the native polyhedrin gene for
blue/white selection. Digestion of BacPAK6 DNA at three sites with Bsu36I
removes lacZ and part of ORF1629, which is an essential AcMNPV gene. The linear virus DNA is unable to initiate
a replication cycle in insect cells. Cotransfection of insect cells with
linear BacPAK6 DNA and a compatible transfer vector containing the complete
ORF1629 and a foreign gene of interest results in recombination and restoration
of the circular, infectious virus genome.
Over 95% of the virus derived from the cotransfection comprises
recombinant virus. Usually, a single
plaque assay titration incubated with X-gal and counterstained with neutral
red is sufficient to differentiate parental blue from recombinant white
plaques. These can be readily isolated
and amplified to working stocks in a few days. The BacPAK6 reagent from OET
Ltd comprises transfection-ready linearised (Bsu36I-digested) virus DNA. |
|
|
|
|
|
|
|
|
|
|
O-101101 |
BacPAK6 Linearised DNA |
5 reacts kit |
on quote |
|
|
O-101102 |
BacPAK6 Linearised DNA |
24 reacts kit |
on quote |
|
|
O-101103 |
BacPAK6 Linearised DNA |
96 reacts kit |
on quote |
|
|
|
|
|
|
|
|
O-101104 |
BacPAK6 sec+ Linearised DNA |
5 reacts kit |
on quote |
|
|
O-101105 |
BacPAK6 sec+ Linearised DNA |
24 reacts kit |
on quote |
|
|
O-101106 |
BacPAK6 sec+ Linearised DNA |
96 reacts kit |
on quote |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Production of recombinant Baculoviruses
Expression Vectors |
|
|
|
|
|
|
|
|
Article No. |
Description |
Size |
Price |
|
|
|
|
|
|
|
|
|
flashBAC |
|
|
|
|
|
For the production of
recombinant baculoviruses. Specifically designed to remove the need to
separate recombinant virus from parental virus by plaque-purification or any
other means. The production of recombinant virus has been reduced to a
one-step procedure in insect cells and
is thus fully amenable to high throughput and automated production systems.
Baculovirus genomes contain several auxillary genes, which are non-essential
for replication in insect cell culture. One of these is chitinase (chiA),
which encodes an enzyme with exo- and endo chitinase activity. In an infected
insect, chitinase (together with cathepsin) facilitates host cuticle
breakdown and tissue liquefaction at the very late stages of infection, so
releasing the virus to infect more hosts.Confocal and electron microscopy
observations of insect cells infected with AcMNPV have shown that chitinase
is targeted to the endoplasmic reticulum where it is densely packed in a
paracrystalline array, severely compromising the function and efficacy of the
secretory pathway. Deletion of chiA from flashBAC™ has improved the efficacy
ofthe secretory pathway and resulted in a greatly enhanced (up to 60-fold in
some instances) yield of recombinant proteins. This one-step procedure
greatly facilitates the high throughput production of baculovirus expression
vectors via automated systems. However, it is also of benefit to the small
research group just requiring one or a few recombinant baculoviruses prepared
in individual dishes of cells.
|
|
|
|
|
|
|
|
|
|
|
O-100150 |
flashBAC |
5 reacts |
on quote |
|
|
O-100151 |
flashBAC |
24 reacts |
on quote |
|
|
O-100152 |
flashBAC |
96 reacts |
on quote |
|
|
O-100153 |
flashBAC |
Bulk |
on quote |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Article No. |
Description |
Size |
Price |
|
|
|
|
|
|
|
|
|
flashBAC GOLD |
|
|
|
|
|
flashBACGOLD™ provides
superior levels of expression for any protein that is destined for secretion
or to be inserted in the membrane, or for any protein that might be
particularly liable to degradation. flashBACGOLD™ is a baculovirus expression
vector that has been designed to reduce proteolysis, maximise protein
secretion and improve membrane protein targeting and is based on our patented
flashBAC™ system, removing the necessity for plaque-purification. The
deletion of both chiA and v-cath genes from flashBACGOLD™ has improved the
efficacy of the secretory pathway and resulted in a greatly enhanced yield of
recombinant proteins that are secreted or membrane targeted (in comparison to
recombinant viruses that encode chiA and v-cath). v-cath encodes a cysteine
protease. Therefore deletion of this gene results in the significant
reduction of degradation of protease-sensitive targets. It is also
back-compatible with all existing baculovirus transfer vectors based on
homologous recombination in insect cells at the polyhedrin locus. |
|
|
|
|
|
|
|
|
|
|
O-100200 |
flashBAC GOLD |
3 reacts |
on quote |
|
|
O-100201 |
flashBAC GOLD |
5 reacts |
on quote |
|
|
O-100202 |
flashBAC GOLD |
24 reacts |
on quote |
|
|
O-100203 |
flashBAC GOLD |
96 reacts |
on quote |
|
|
O-100204 |
flashBAC GOLD |
Bulk |
on quote |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Article No. |
Description |
Size |
Price |
|
|
|
|
|
|
|
|
|
flashBAC ULTRA |
|
|
|
|
|
flashBACULTRA™ has taken to
system a step further, by the removal of three more virus genes (p10, p74 and
p26) from the flashBACULTRA™ genome. This has allowed further improvements in
recombinant protein yield and quality to be delivered. Deletion of p10
increases polh activity providing more recombinant protein, increases nuclear
and cellular stability, ensuring a longer timeframe for protein expression
and removes a major competitor for limiting cellular resources. p74 is
non-essential in cell culture but is essential for oral infectivity of
occlusion-derived virus (ODV) in the host where it plays a role in midgut
attachment and fusion. Deletion of p74 has been shown to have no effect on
virus production in vitro. Deletion of p74 further increases the biosafety
profile of recombinant baculoviruses in the environment, making them unable
to traverse the insect gut wall. P26 is an early gene that codes for a
240-amino acid polypeptide of unknown function and has the same 5′
terminus as p10. Deletion of the 3’-end of p26 and fusion to lacZ or p10 have
previously been shown to have no effect on virus replication in vitro.
Deletion of p10, p74 and p26 removes an unnecessary genetic burden from the
recombinant virus genome, providing a more efficient baculovirus expression
vector. Taken together with the chitinase and cathepsin deletions this makes
flashBAC ULTRA the best choice for the most difficult to express proteins.
However flashBAC ULTRA is suitable for the expression of cytoplasmic, nucleus,
secreted and membrane proteins. |
|
|
|
|
|
|
|
|
|
|
O-100304 |
flashBAC ULTRA |
3 reacts |
on quote |
|
|
O-100300 |
flashBAC ULTRA |
5 reacts |
on quote |
|
|
O-100301 |
flashBAC ULTRA |
24 reacts |
on quote |
|
|
O-100302 |
flashBAC ULTRA |
96 reacts |
on quote |
|
|
O-100303 |
flashBAC ULTRA |
Bulk |
on quote |
|
|
|
|
|
|
|
|
O-100400 |
flashBAC selection box
1. (Includes flashBAC, flashBAC GOLD
& flashBAC ULTRA) |
3 x 3 reacts |
on quote |
|
|
O-100401 |
flashBAC selection box 2.
(Includes flashBAC PRIME, flashBAC GOLD & flashBAC ULTRA) |
4 x 3 reacts |
on quote |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Article No. |
Description |
Size |
Price |
|
|
|
|
|
|
|
|
|
flashBAC PRIME |
|
|
|
|
|
Designed with virus like
particle production in mind, flashBAC PRIME combines the simplicity and easy
to use flashBAC one step baculovirus expression system with increased
recovery and yield of virus like particles. Although focused on virus like
particle production, flashBAC PRIME is also well suited to cytoplasmic
protein expression and production. For best results when expressing virus
like particles we recommend using the recombinant virus in Trichoplusia ni
(Tni) derived cell lines. The one-step procedure offered by all varients of
flashBAC greatly facilitates the high throughput production of baculovirus
expression vectors via automated systems. However, it is also of benefit to
the small research group just requiring one or a few recombinant baculoviruses
prepared in individual dishes of cells. |
|
|
|
|
|
|
|
|
|
|
O-100500 |
flashBAC PRIME |
5 reacts |
on quote |
|
|
O-100501 |
flashBAC PRIME |
24 reacts |
on quote |
|
|
O-100502 |
flashBAC PRIME |
Bulk |
on quote |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Baculovirus Protein
Expression Kits |
|
|
|
|
|
|
|
|
|
|
Article No. |
Description |
Size |
Price |
|
|
|
|
|
|
|
|
O-400100 |
baculoCOMPLETE protein expression kit |
5 reacts |
on quote |
|
|
O-400101 |
baculoCOMPLETE protein expression kit + baculoQUANT
all-in-one |
5 + 100 reacts |
on quote |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Virus Titration |
|
|
|
|
|
|
|
|
|
|
Article No. |
Description |
Size |
Price |
|
|
|
|
|
|
|
|
O-100602 |
baculoQUANT All-in-one
virus extraction & titration kit |
100 reacts |
on quote |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Transfer Plasmids |
|
|
|
|
|
The pOET1 and pOET2
plasmids are designed to allow simple, easy cloning into the baculovirus
system. The pOET3 and pOET4 plasmids are designed for improved expression of
glycosylated, secreted and membrane-targeted proteins and are an ideal
companion to the flashBAC GOLD and ULTRA products. |
|
|
|
|
|
|
|
|
|
|
Article No. |
Description |
Size |
Price |
|
|
|
|
|
|
|
|
O-200101 |
pOET1.1 transfer plasmid |
10 ug |
on quote |
|
|
O-2001011 |
pOET1.1N_6xHis transfer plasmid |
10 ug |
on quote |
|
|
O-2001012 |
pOET1.1C_6xHis transfer plasmid |
10 ug |
on quote |
|
|
O-200103 |
pOET2.1 transfer plasmid |
10 ug |
on quote |
|
|
O-2001031 |
pOET2.1N/C_6xHis transfer plasmid |
10 ug |
on quote |
|
|
O-2001032 |
pOET2.1C_6xHis transfer plasmid |
10 ug |
on quote |
|
|
O-200104 |
pOET3 transfer plasmid |
10 ug |
on quote |
|
|
O-200105 |
pOET4 transfer plasmid |
10 ug |
on quote |
|
|
O-200106 |
pOET5.1 transfer plasmid |
10 ug |
on quote |
|
|
|
|
|
|
|
|
O-200107 |
pOET6 BacMAM transfer plasmid |
10 ug |
on quote |
|
|
|
|
|
|
|
|
O-200121 |
pOET8.VE1 transfer plasmid |
10 µg |
on quote |
|
|
O-200122 |
pOET8.VE2 transfer plasmid |
10 µg |
on quote |
|
|
O-200123 |
pOET8.VE3 transfer plasmid |
10 µg |
on quote |
|
|
|
|
|
|
|
|
O-200131 |
pOET9 EF1alpha transfer plasmid |
10 µg |
on quote |
|
|
O-200132 |
pOET9 CCAG transfer plasmid |
10 µg |
on quote |
|
|
O-200133 |
pOET9 CMV transfer plasmid |
10 µg |
on quote |
|
|
O-200134 |
pOET SV40 transfer plasmid |
10 µg |
on quote |
|
|
|
|
|
|
|
|
O-200135 |
pOET Selection Box |
4 x 10 µg |
on quote |
|
|
|
|
|
|
|
|
|
Transfection Reagent |
|
|
|
|
|
|
|
|
|
|
Article No. |
Description |
Size |
Price |
|
|
|
|
|
|
|
|
O-300105 |
baculoFECTIN II |
150ul |
on quote |
|
|
O-300106 |
baculoFECTIN II |
1ml |
on quote |
|
|
|
|
|
|
|
|
|
Insect Cell Culture Media |
|
|
|
|
|
|
|
|
|
|
Article No. |
Description |
Size |
Price |
|
|
|
|
|
|
|
|
O-500300 |
ESF921 protein free insect cell culture medium |
1000 ml |
on quote |
|
|
|
|
|
|
|
|
O-500306 |
Expression Systems custom made media |
min 20 Ltr |
on quote |
|
|
|
|
|
|
|
|
O-500307 |
Production Boost Additive |
100 ml |
on request |
|
|
O-500308 |
Transfection Media |
20 ml |
on request |
|
|
O-500309 |
Transfection Media |
100 ml |
on request |
|
|
O-500310 |
ESF921 delta series methionine deficient insect cell
culture medium |
1000 ml |
on request |
|
|
O-500311 |
ESF921 delta series all amino acids deficient insect cell
culture medium |
1000 ml |
on request |
|
|
O-500400 |
ESF Animal Free Insect Cell Media |
1000 ml |
on request |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Insect Cells |
|
|
|
|
|
|
|
|
|
|
Article No. |
Description |
Size |
Price |
|
|
|
|
|
|
|
|
O-600100 |
Sf9 insect cells (1x10e7 cells) |
2 vials |
on quote |
|
|
O-600105 |
Sf21 (plaque assay) insect
cells (1x10e7 cells) |
2 vials |
on quote |
|
|
|
|
|
|
|
|
O-600102 |
superSf9-1 insect cells
(1x10e7 cells) |
2 vials |
on quote |
|
|
O-600103 |
superSf9-2 insect cells
(1x10e7 cells) |
2 vials |
on quote |
|
|
O-600104 |
superSf9-3 insect
cells (1x10e7 cells) |
2 vials |
on quote |
|
|
|
|
|
|
|
|
|
|
|
|
|
| |
Quoted prices in EUR net, exw + VAT. Pricelist
& prices subject to change without prior notice. |
|
| |
Not addressed to endusers in the legal sense
of price regulation. |
|
|
|
| |
|
|
|
|
|
| |
Copyright ©
CELL CONCEPTS GmbH & GBIOSCIENCES Inc. 2022 |
07.01.2022 |
(vs1.22) |
|
|
|
|
|
|
|
|
|
|
|
|
|