Baculovirus Expression Technology
  2022   PRICE LIST     -   CELL CONCEPTS  /  OXFORD EXPRESSION TECHNOLOGIES    
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Genenerating recombinant Baculovirus Expression Vectors
Article No. Description Size Price
BacPAK6
BacPAK6 linear DNA is the original convenient, highly efficient reagent for generating recombinant baculovirus expression vectors.  It comprises a modified Autographa californica nucleopolyhedrovirus AcMNPV) genome with lacZ inserted in place of the native polyhedrin gene for blue/white selection. Digestion of BacPAK6 DNA at three sites with Bsu36I removes lacZ and part of ORF1629, which is an essential AcMNPV gene.  The linear virus DNA is unable to initiate a replication cycle in insect cells. Cotransfection of insect cells with linear BacPAK6 DNA and a compatible transfer vector containing the complete ORF1629 and a foreign gene of interest results in recombination and restoration of the circular, infectious virus genome.  Over 95% of the virus derived from the cotransfection comprises recombinant virus.  Usually, a single plaque assay titration incubated with X-gal and counterstained with neutral red is sufficient to differentiate parental blue from recombinant white plaques.  These can be readily isolated and amplified to working stocks in a few days. The BacPAK6 reagent from OET Ltd comprises transfection-ready linearised (Bsu36I-digested) virus DNA. 
O-101101 BacPAK6 Linearised DNA 5 reacts kit on quote
O-101102 BacPAK6 Linearised DNA 24 reacts kit on quote
O-101103 BacPAK6 Linearised DNA 96 reacts kit on quote
O-101104 BacPAK6 sec+ Linearised DNA 5 reacts kit on quote
O-101105 BacPAK6 sec+ Linearised DNA 24 reacts kit on quote
O-101106 BacPAK6 sec+ Linearised DNA 96 reacts kit on quote
 Production of recombinant Baculoviruses Expression Vectors
Article No. Description Size Price
flashBAC
For the production of recombinant baculoviruses. Specifically designed to remove the need to separate recombinant virus from parental virus by plaque-purification or any other means. The production of recombinant virus has been reduced to a one-step procedure  in insect cells and is thus fully amenable to high throughput and automated production systems. Baculovirus genomes contain several auxillary genes, which are non-essential for replication in insect cell culture. One of these is chitinase (chiA), which encodes an enzyme with exo- and endo chitinase activity. In an infected insect, chitinase (together with cathepsin) facilitates host cuticle breakdown and tissue liquefaction at the very late stages of infection, so releasing the virus to infect more hosts.Confocal and electron microscopy observations of insect cells infected with AcMNPV have shown that chitinase is targeted to the endoplasmic reticulum where it is densely packed in a paracrystalline array, severely compromising the function and efficacy of the secretory pathway. Deletion of chiA from flashBAC™ has improved the efficacy ofthe secretory pathway and resulted in a greatly enhanced (up to 60-fold in some instances) yield of recombinant proteins. This one-step procedure greatly facilitates the high throughput production of baculovirus expression vectors via automated systems. However, it is also of benefit to the small research group just requiring one or a few recombinant baculoviruses prepared in individual dishes of cells.
O-100150 flashBAC 5 reacts on quote
O-100151 flashBAC 24 reacts on quote
O-100152 flashBAC 96 reacts on quote
O-100153 flashBAC Bulk on quote
Article No. Description Size Price
flashBAC GOLD
flashBACGOLD™ provides superior levels of expression for any protein that is destined for secretion or to be inserted in the membrane, or for any protein that might be particularly liable to degradation. flashBACGOLD™ is a baculovirus expression vector that has been designed to reduce proteolysis, maximise protein secretion and improve membrane protein targeting and is based on our patented flashBAC™ system, removing the necessity for plaque-purification. The deletion of both chiA and v-cath genes from flashBACGOLD™ has improved the efficacy of the secretory pathway and resulted in a greatly enhanced yield of recombinant proteins that are secreted or membrane targeted (in comparison to recombinant viruses that encode chiA and v-cath). v-cath encodes a cysteine protease. Therefore deletion of this gene results in the significant reduction of degradation of protease-sensitive targets. It is also back-compatible with all existing baculovirus transfer vectors based on homologous recombination in insect cells at the polyhedrin locus.
O-100200 flashBAC GOLD 3 reacts on quote
O-100201 flashBAC GOLD 5 reacts on quote
O-100202 flashBAC GOLD 24 reacts on quote
O-100203 flashBAC GOLD 96 reacts on quote
O-100204 flashBAC GOLD Bulk on quote
Article No. Description Size Price
flashBAC ULTRA
flashBACULTRA™ has taken to system a step further, by the removal of three more virus genes (p10, p74 and p26) from the flashBACULTRA™ genome. This has allowed further improvements in recombinant protein yield and quality to be delivered. Deletion of p10 increases polh activity providing more recombinant protein, increases nuclear and cellular stability, ensuring a longer timeframe for protein expression and removes a major competitor for limiting cellular resources. p74 is non-essential in cell culture but is essential for oral infectivity of occlusion-derived virus (ODV) in the host where it plays a role in midgut attachment and fusion. Deletion of p74 has been shown to have no effect on virus production in vitro. Deletion of p74 further increases the biosafety profile of recombinant baculoviruses in the environment, making them unable to traverse the insect gut wall. P26 is an early gene that codes for a 240-amino acid polypeptide of unknown function and has the same 5′ terminus as p10. Deletion of the 3’-end of p26 and fusion to lacZ or p10 have previously been shown to have no effect on virus replication in vitro. Deletion of p10, p74 and p26 removes an unnecessary genetic burden from the recombinant virus genome, providing a more efficient baculovirus expression vector. Taken together with the chitinase and cathepsin deletions this makes flashBAC ULTRA the best choice for the most difficult to express proteins. However flashBAC ULTRA is suitable for the expression of cytoplasmic, nucleus, secreted and membrane proteins.
O-100304 flashBAC ULTRA 3 reacts on quote
O-100300 flashBAC ULTRA 5 reacts on quote
O-100301 flashBAC ULTRA 24 reacts on quote
O-100302 flashBAC ULTRA 96 reacts on quote
O-100303 flashBAC ULTRA Bulk on quote
O-100400 flashBAC selection box 1.  (Includes flashBAC, flashBAC GOLD & flashBAC ULTRA) 3 x 3 reacts on quote
O-100401 flashBAC selection box 2. (Includes flashBAC PRIME, flashBAC GOLD & flashBAC ULTRA) 4 x 3 reacts on quote
Article No. Description Size Price
flashBAC PRIME
Designed with virus like particle production in mind, flashBAC PRIME combines the simplicity and easy to use flashBAC one step baculovirus expression system with increased recovery and yield of virus like particles. Although focused on virus like particle production, flashBAC PRIME is also well suited to cytoplasmic protein expression and production. For best results when expressing virus like particles we recommend using the recombinant virus in Trichoplusia ni (Tni) derived cell lines. The one-step procedure offered by all varients of flashBAC greatly facilitates the high throughput production of baculovirus expression vectors via automated systems. However, it is also of benefit to the small research group just requiring one or a few recombinant baculoviruses prepared in individual dishes of cells.
O-100500 flashBAC PRIME 5 reacts on quote
O-100501 flashBAC PRIME 24 reacts on quote
O-100502 flashBAC PRIME Bulk on quote
Baculovirus Protein Expression Kits
Article No. Description Size Price
O-400100 baculoCOMPLETE protein expression kit 5 reacts on quote
O-400101 baculoCOMPLETE protein expression kit + baculoQUANT all-in-one 5 + 100 reacts on quote
Virus Titration
Article No. Description Size Price
O-100602 baculoQUANT All-in-one virus extraction & titration kit 100 reacts on quote
Transfer Plasmids
The pOET1 and pOET2 plasmids are designed to allow simple, easy cloning into the baculovirus system. The pOET3 and pOET4 plasmids are designed for improved expression of glycosylated, secreted and membrane-targeted proteins and are an ideal companion to the flashBAC GOLD and ULTRA products. 
Article No. Description Size Price
O-200101 pOET1.1 transfer plasmid 10 ug on quote
O-2001011 pOET1.1N_6xHis transfer plasmid 10 ug on quote
O-2001012 pOET1.1C_6xHis transfer plasmid 10 ug on quote
O-200103 pOET2.1 transfer plasmid 10 ug on quote
O-2001031 pOET2.1N/C_6xHis transfer plasmid 10 ug on quote
O-2001032 pOET2.1C_6xHis transfer plasmid 10 ug on quote
O-200104 pOET3 transfer plasmid 10 ug on quote
O-200105 pOET4 transfer plasmid 10 ug on quote
O-200106 pOET5.1 transfer plasmid 10 ug on quote
O-200107 pOET6 BacMAM transfer plasmid 10 ug on quote
O-200121 pOET8.VE1 transfer plasmid 10 µg on quote
O-200122 pOET8.VE2 transfer plasmid 10 µg on quote
O-200123 pOET8.VE3 transfer plasmid 10 µg on quote
O-200131 pOET9 EF1alpha transfer plasmid 10 µg on quote
O-200132 pOET9 CCAG transfer plasmid 10 µg on quote
O-200133 pOET9 CMV transfer plasmid 10 µg on quote
O-200134 pOET SV40 transfer plasmid 10 µg on quote
O-200135 pOET Selection Box 4 x 10 µg on quote
Transfection Reagent
Article No. Description Size Price
O-300105 baculoFECTIN II 150ul on quote
O-300106 baculoFECTIN II 1ml on quote
Insect Cell Culture Media
Article No. Description Size Price
O-500300 ESF921 protein free insect cell culture medium 1000 ml on quote
O-500306 Expression Systems custom made media min 20 Ltr on quote
O-500307 Production Boost Additive 100 ml on request
O-500308 Transfection Media 20 ml on request
O-500309 Transfection Media 100 ml on request
O-500310 ESF921 delta series methionine deficient insect cell culture medium 1000 ml on request
O-500311 ESF921 delta series all amino acids deficient insect cell culture medium 1000 ml on request
O-500400 ESF Animal Free Insect Cell Media 1000 ml on request
Insect Cells
Article No. Description Size Price
O-600100 Sf9 insect cells  (1x10e7 cells) 2 vials on quote
O-600105 Sf21 (plaque assay) insect cells  (1x10e7 cells) 2 vials on quote
O-600102 superSf9-1 insect cells  (1x10e7 cells) 2 vials on quote
O-600103 superSf9-2 insect cells  (1x10e7 cells) 2 vials on quote
O-600104 superSf9-3 insect cells  (1x10e7 cells) 2 vials on quote
  Quoted prices in EUR net, exw + VAT. Pricelist & prices subject to change without prior notice.  
  Not addressed to endusers in the legal sense of price regulation.      
           
  Copyright ©  CELL CONCEPTS GmbH   &  GBIOSCIENCES Inc.  2022 07.01.2022 (vs1.22)